Last data update: May 06, 2024. (Total: 46732 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Chotiwan N[original query] |
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Rapid and specific detection of Asian- and African-lineage Zika viruses.
Chotiwan N , Brewster CD , Magalhaes T , Weger-Lucarelli J , Duggal NK , Ruckert C , Nguyen C , Garcia Luna SM , Fauver JR , Andre B , Gray M , Black WCth , Kading RC , Ebel GD , Kuan G , Balmaseda A , Jaenisch T , Marques ETA , Brault AC , Harris E , Foy BD , Quackenbush SL , Perera R , Rovnak J . Sci Transl Med 2017 9 (388) Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers. |
Molecular determinants of dengue virus 2 envelope protein important for virus entry in Fc?RIIA-mediated antibody-dependent enhancement of infection.
Chotiwan N , Roehrig JT , Schlesinger JJ , Blair CD , Huang CYH . Virology 2014 456-457 (1) 238-246 Antibody-dependent enhancement (ADE) of infection may cause severe illness in patients suffering a secondary infection by a heterologous dengue virus (DENV) serotype. During ADE of infection, cross-reactive non- or poorly-neutralizing antibodies form infectious virus-Ab complexes with the newly infecting serotype and enhance virus infection by binding to the Fc receptors (FcR) on FcR-bearing cells. In this study, we determined that molecular determinants of DENV2 envelope protein critical for virus entry during non-ADE infection are also required for ADE infection mediated by FcRIIA, and binding of virus-Ab complexes with FcRIIA alone is not sufficient for ADE of infection. The FcRIIA mainly plays an auxiliary role in concentrating the virus-Ab complex to the cell surface, and other primary cellular receptors are required for virus entry. Understanding the viral entry pathway in ADE of DENV infection will greatly facilitate rational designs of anti-viral therapeutics against severe dengue disease associated with ADE. |
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